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cd3 alexafluor 405  (Bio-Techne corporation)


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    Bio-Techne corporation cd3 alexafluor 405
    Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare <t>CD3</t> and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.
    Cd3 Alexafluor 405, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd3 alexafluor 405/product/Bio-Techne corporation
    Average 90 stars, based on 3 article reviews
    cd3 alexafluor 405 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Feeder-Cell-Free and Serum-Free Expansion of Natural Killer Cells Using Cloudz Microspheres, G-Rex6M, and Human Platelet Lysate"

    Article Title: Feeder-Cell-Free and Serum-Free Expansion of Natural Killer Cells Using Cloudz Microspheres, G-Rex6M, and Human Platelet Lysate

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.803380

    Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare CD3 and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.
    Figure Legend Snippet: Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare CD3 and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.

    Techniques Used: Cell Culture, Flow Cytometry, Expressing

    Comparison of the xeno-free 10% GMP human platelet lysate/NK Cloudz protocol to the existing FBS/NK Cloudz protocol in the G-Rex6M. The results show (A) the cell distribution on day 10, (B) NK (CD3 − CD56 + ) fold change between days 0 and 10, (C) the viability of either all CD45 + cells or NK cells alone on day 10, and (D) the percent of target K562 cells killed on day 10 with a 1:1 effector:target (E:T) ratio and a 4 h incubation. Data represent the mean ± SD from 4 separate donors. The GMP human platelet lysate resulted in similar purity, expansion, viability, and cytotoxicity as the FBS protocol.
    Figure Legend Snippet: Comparison of the xeno-free 10% GMP human platelet lysate/NK Cloudz protocol to the existing FBS/NK Cloudz protocol in the G-Rex6M. The results show (A) the cell distribution on day 10, (B) NK (CD3 − CD56 + ) fold change between days 0 and 10, (C) the viability of either all CD45 + cells or NK cells alone on day 10, and (D) the percent of target K562 cells killed on day 10 with a 1:1 effector:target (E:T) ratio and a 4 h incubation. Data represent the mean ± SD from 4 separate donors. The GMP human platelet lysate resulted in similar purity, expansion, viability, and cytotoxicity as the FBS protocol.

    Techniques Used: Comparison, Incubation

    The G-Rex6M/NK Cloudz™ “low-touch” protocol compared with the flask-based “high-touch” feeder cell protocol. The feeder cell protocol used the same donors but was otherwise different: (See methods in (A) or in the Materials and Methods section). The results show the following: (B) the cell distribution on day 10, (C) the NK (CD3 − CD56 + ) fold change between days 0 and 10, (D) the viability of either all CD45 + cells (light gray bars) or NK cells alone (dark gray bars) on day 10, and (E) the percent of target K562 cells killed with a 1:1 effector:target (E:T) ratio over 4 h. Data represent the mean ± SD from 3 separate donors.
    Figure Legend Snippet: The G-Rex6M/NK Cloudz™ “low-touch” protocol compared with the flask-based “high-touch” feeder cell protocol. The feeder cell protocol used the same donors but was otherwise different: (See methods in (A) or in the Materials and Methods section). The results show the following: (B) the cell distribution on day 10, (C) the NK (CD3 − CD56 + ) fold change between days 0 and 10, (D) the viability of either all CD45 + cells (light gray bars) or NK cells alone (dark gray bars) on day 10, and (E) the percent of target K562 cells killed with a 1:1 effector:target (E:T) ratio over 4 h. Data represent the mean ± SD from 3 separate donors.

    Techniques Used:



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    Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare <t>CD3</t> and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.
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    anti-cd3-alexafluor 405  (Thermo Fisher)
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    Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare <t>CD3</t> and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.
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    Image Search Results


    Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare CD3 and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.

    Journal: Frontiers in Immunology

    Article Title: Feeder-Cell-Free and Serum-Free Expansion of Natural Killer Cells Using Cloudz Microspheres, G-Rex6M, and Human Platelet Lysate

    doi: 10.3389/fimmu.2022.803380

    Figure Lengend Snippet: Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare CD3 and CD56 expression of the expanded cell population. Black arrows indicate CD3 − CD56 + (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45 + cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45 + cells (including NK cells) and the dark gray bars represent the viability of only the CD45 + CD3 − CD56 + (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.

    Article Snippet: Samples were washed with flow buffer (PBS supplemented with 1% BSA (BP9700-100, ThermoFisher)), then stained with CD3-AlexaFluor 405 (FAB100V, Bio-Techne), CD56-APC (318310, Biolegend, San Diego, CA, USA), CD45-PE-cy7 (557748, BD Biosciences, Franklin Lakes, NJ, USA), CD16-PerCP (302030, Biolegend), NKp46-BB515 (564536, BD), NKG2D-PE (557940, BD), and CD2-AlexaFluor750 (FAB18561S, Bio-Techne).

    Techniques: Cell Culture, Flow Cytometry, Expressing

    Comparison of the xeno-free 10% GMP human platelet lysate/NK Cloudz protocol to the existing FBS/NK Cloudz protocol in the G-Rex6M. The results show (A) the cell distribution on day 10, (B) NK (CD3 − CD56 + ) fold change between days 0 and 10, (C) the viability of either all CD45 + cells or NK cells alone on day 10, and (D) the percent of target K562 cells killed on day 10 with a 1:1 effector:target (E:T) ratio and a 4 h incubation. Data represent the mean ± SD from 4 separate donors. The GMP human platelet lysate resulted in similar purity, expansion, viability, and cytotoxicity as the FBS protocol.

    Journal: Frontiers in Immunology

    Article Title: Feeder-Cell-Free and Serum-Free Expansion of Natural Killer Cells Using Cloudz Microspheres, G-Rex6M, and Human Platelet Lysate

    doi: 10.3389/fimmu.2022.803380

    Figure Lengend Snippet: Comparison of the xeno-free 10% GMP human platelet lysate/NK Cloudz protocol to the existing FBS/NK Cloudz protocol in the G-Rex6M. The results show (A) the cell distribution on day 10, (B) NK (CD3 − CD56 + ) fold change between days 0 and 10, (C) the viability of either all CD45 + cells or NK cells alone on day 10, and (D) the percent of target K562 cells killed on day 10 with a 1:1 effector:target (E:T) ratio and a 4 h incubation. Data represent the mean ± SD from 4 separate donors. The GMP human platelet lysate resulted in similar purity, expansion, viability, and cytotoxicity as the FBS protocol.

    Article Snippet: Samples were washed with flow buffer (PBS supplemented with 1% BSA (BP9700-100, ThermoFisher)), then stained with CD3-AlexaFluor 405 (FAB100V, Bio-Techne), CD56-APC (318310, Biolegend, San Diego, CA, USA), CD45-PE-cy7 (557748, BD Biosciences, Franklin Lakes, NJ, USA), CD16-PerCP (302030, Biolegend), NKp46-BB515 (564536, BD), NKG2D-PE (557940, BD), and CD2-AlexaFluor750 (FAB18561S, Bio-Techne).

    Techniques: Comparison, Incubation

    The G-Rex6M/NK Cloudz™ “low-touch” protocol compared with the flask-based “high-touch” feeder cell protocol. The feeder cell protocol used the same donors but was otherwise different: (See methods in (A) or in the Materials and Methods section). The results show the following: (B) the cell distribution on day 10, (C) the NK (CD3 − CD56 + ) fold change between days 0 and 10, (D) the viability of either all CD45 + cells (light gray bars) or NK cells alone (dark gray bars) on day 10, and (E) the percent of target K562 cells killed with a 1:1 effector:target (E:T) ratio over 4 h. Data represent the mean ± SD from 3 separate donors.

    Journal: Frontiers in Immunology

    Article Title: Feeder-Cell-Free and Serum-Free Expansion of Natural Killer Cells Using Cloudz Microspheres, G-Rex6M, and Human Platelet Lysate

    doi: 10.3389/fimmu.2022.803380

    Figure Lengend Snippet: The G-Rex6M/NK Cloudz™ “low-touch” protocol compared with the flask-based “high-touch” feeder cell protocol. The feeder cell protocol used the same donors but was otherwise different: (See methods in (A) or in the Materials and Methods section). The results show the following: (B) the cell distribution on day 10, (C) the NK (CD3 − CD56 + ) fold change between days 0 and 10, (D) the viability of either all CD45 + cells (light gray bars) or NK cells alone (dark gray bars) on day 10, and (E) the percent of target K562 cells killed with a 1:1 effector:target (E:T) ratio over 4 h. Data represent the mean ± SD from 3 separate donors.

    Article Snippet: Samples were washed with flow buffer (PBS supplemented with 1% BSA (BP9700-100, ThermoFisher)), then stained with CD3-AlexaFluor 405 (FAB100V, Bio-Techne), CD56-APC (318310, Biolegend, San Diego, CA, USA), CD45-PE-cy7 (557748, BD Biosciences, Franklin Lakes, NJ, USA), CD16-PerCP (302030, Biolegend), NKp46-BB515 (564536, BD), NKG2D-PE (557940, BD), and CD2-AlexaFluor750 (FAB18561S, Bio-Techne).

    Techniques: